Antimycobacterial activity of aqueous and methanol extracts of nine plants against Mycobacterium bacteria


 Purpose: The present study was done to evaluate the antimycobacterial activity of aqueous and methanol extracts nine plants viz., Buddleja saligna, Carpobrotus dimidiatus, Capparis tomentosa, Dichrostachys cinerea, Ekebergia capensis, Ficus sur, Gunnera perpensa, Leonotis leonurus Tetradenia riparia in South Africa.
 Methods: Aqueous leaves were tested vitro for their against Mycobacterium smegmatis, tuberculosis H37Rv (ATCC 25177) three well-characterized clinical isolates MDR XDR-TB using agar incorporation method. minimum inhibitory concentration (MIC) each active plant extract determined broth microdilution Cytotoxic effect evaluated mouse BALB/C monocyte macrophage (J774.2) peripheral blood mononuclear cells (PBMCs) whole toxicity screening brine shrimp lethality assay. Composition thin layer chromatography while qualitative analysis compounds TLC-Bioautography
 Results: B. C. tormentosa dimidiatus; G. perpensa T. possessed significant M. XDR-TB. Except a high none other which showed any toxic or cytotoxic activity.
 Conclusion: Our findings show that tormentosa, have activity. Further studies would aim at isolation identification from had positive